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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Histone H2A.V,H2AFV
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Histone H2A.V, H2AFV ELISA KIT
ELI-19382c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Histone H2A.V, H2AFV ELISA KIT is for the quantitative in vitro determination of Chicken H2AFV concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Histone H2A.V, H2AFV ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken H2AFV in the sample, this Chicken Histone H2A.V, H2AFV ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken H2AFV concentration. The concentration of Chicken H2AFV in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验for the purification of nuclei and for the preparation of input chromatin. Sodium butyrate prevents loss of histone acetylation via the nonspecific action of endogenous histone deacetylases. Sodium dodecyl sulfate (SDS; 10 %, w/v) TBE buffer (1X
染色质的基本结构单位,为核蛋白的结构体。亦称 v体( v body)。核小体为由组蛋白 H2A、 H2B、 H3及 H4各 2分子所形成的组蛋白 8聚体( histone octamer),即在组蛋白核心( histoncore)的周围绕以 DNA。用某种核酸酶处理可分成核小体核心粒子( nucleosome core particle)和具有核粒子间连接作用的连接 DNA( Linker DNA)二部分。核心粒子是由组蛋白核心和绕在周围的有 146个核苷酸对的 DNA所构成
-H2A. X/v的同时告诉细胞这个缺陷已经被成功修复了。 这些发现回答了有关DNA双链缺口修复的基本问题并最终可能促进找出一些癌症治疗的新策略。而且,Kusch博士还将继续深入研究dTip60复合体以期彻底弄清这个重要的生物过程。 原文摘要: Acetylation by Tip60 Is Required for Selective Histone Variant Exchange at DNA Lesions
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