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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Myelin protein P0,MPZ
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Myelin protein P0, MPZ ELISA KIT
ELI-16641c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Myelin protein P0, MPZ ELISA KIT is for the quantitative in vitro determination of Chicken MPZ concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Myelin protein P0, MPZ ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken MPZ in the sample, this Chicken Myelin protein P0, MPZ ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken MPZ concentration. The concentration of Chicken MPZ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验with 250 µl Pierce Coomassie protein assay reagent in 96-well ELISA dishes and reading at 650 nm on a plate reader).The foregoing affinity purification steps were carried out at 4ºC.The proteins were dialyzed against PBS and stored at –20ºC.Protein
Antigen Design Sera Purification Tech Sheet
to recognize that the immune system reacts to the peptide-protein carrier as a whole and that there will be a portion of response directed against the conjugated peptide as well as the linker and the carrier protein1. When screening by ELISA it is advisable
对肝纤维化的发生、发展发挥重要作用呢?我们课题组的先前研究表明在肝纤维大鼠的肝组织中HMGB1的表达明显增强,但HMGB1是否通过激活HSC参与肝纤维化的发生发展目前尚不清楚,本研究拟通过HMGB1刺激HSC以观察ECM的合成情况来进一步探讨HMGB1与肝纤维化的关系。 1 材料与方法 1.1 主要试剂 HMGB1由本室制备保存;新生胎牛血清(FCS)为杭州四季青生物工程材料研究所产品;高糖型DMEM购自英国Gibco公司;大鼠PⅢP ,CⅣ双抗体夹心ELISA检测试剂盒购自
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