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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Macrophage migration inhibitory factor,MIF
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Macrophage migration inhibitory factor, MIF ELISA KIT
ELI-02431c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Macrophage migration inhibitory factor, MIF ELISA KIT is for the quantitative in vitro determination of Chicken MIF concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Macrophage migration inhibitory factor, MIF ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken MIF in the sample, this Chicken Macrophage migration inhibitory factor, MIF ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken MIF concentration. The concentration of Chicken MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Maintenance of Chicken Embryonic Stem Cells In Vitro
In this chapter, we describe the methods we have used to show that chicken leukemia inhibitory factor (LIF) maintains chicken embryonic stem (ES) cells in an undifferentiated state in culture. Recombinant chicken LIF (rchLIF) was expressed
转移抑制因子 migration inhibitory factor 缩写MIF。是淋巴激活素(lymphokine)之一;是与抗原反应的淋巴球产生的物质,作用于巨噬细胞,具有抑制其运动的活性。这种物质的实质尚不清楚,但在分子量约为7万的蛋白质分区里有这种活性,它也作用于别种动物的巨噬细胞。现在认为,与位于出现迟发过敏性反应的地方积聚单核白血球或者巨噬细胞有关。认为MIF产生于T细胞的说法是有力的,但也有实验报道,认为B细胞产生MIF。
细胞活化因子(MAF) IFN-γ 白细胞 白细胞移动抑制因子(LIF) 招引、聚集白细胞 淋巴细胞 IL-2 淋巴细胞局部浸润、增殖 IFN-γ 增强杀伤功能 皮肤血管 皮肤反应因子(SRF) 增加血管通透性 靶细胞 淋巴毒素(LT) 杀伤靶细胞 MCF: macrophage chemotactic factor MIF: macrophage migration inhibition factor MAF: macrophage activating factor
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