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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Immunoglobulin A
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Immunoglobulin A ELISA Kit
ELA-E0546d
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Immunoglobulin A ELISA Kit is for the quantitative in vitro determination of Chicken IgA concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Immunoglobulin A ELISA Kit is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken IgA in the sample, this Chicken Immunoglobulin A ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken IgA concentration. The concentration of Chicken IgA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Understanding the Immunoglobulin Locus Specificity of Hypermutation
The immunoglobulin (Ig ) genes of B cells are diversified at high rate by point mutations whereas the non-Ig genes of B cells accumulate no or significantly fewer mutations. Ig hypermutations are critical for the affinity maturation
Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System
The secondary immunoglobulin gene diversification processes, somatic hypermutation (SHM), immunoglobulin gene conversion (GCV), and class switch recombination, are important for efficient humoral immune responses. They require the action
抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类,即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链(L)和两个重链(H)的单体组成。Ig 的轻链是相同的,有 κ(kappa)和 λ(Lambda)两种型别。五类 Ig 的重链结构不同,这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ(gamma)链和 μ(mu )链。 重链和轻链的 N 端的氨基酸排列顺序
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