- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66060c
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken 25-OH Vitamin D,25OHVD
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken 25- OH Vitamin D ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
25-OH Vitamin D,
Search name
Chicken 25-OH Vitamin D ELISA Kit, 25-OH Vitamin D ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of 25-oh vitamin d,25-OH Vitamin D concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 25-OH Vitamin D, During the reaction, 25-OH Vitamin D in the sample or standard competes with a
fixed amount of biotin-labeled 25-OH Vitamin D for sites on a pre-coated Monoclonal antibody specific to 25-OH Vitamin D. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of 25-OH Vitamin D in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验人25羟基维生素D(25-OH-D)酶联免疫分析（ELISA）
人 25 羟基维生素 D(25 - OH - D ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 25羟基维生素D(25 - OH - D) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 25 羟基维生素 D(25 - OH - D ) 水平。用纯化的 抗 D(25 - OH - D ) 抗体包被微孔板，制成固相
Quantitation of 25-OH-Vitamin D (25OHD) Using Liquid Tandem Mass Spectrometry (LC-MS-MS)
25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids
There are many different methods to choose for vitamin D analysis. While immunoassays are available commercially and readily automated, specificity for 25-hydroxyvitamin D2 and D3 is variable. Chemical assays such as HPLC and LC
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