- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66032c
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken S-Adenosyl Methionine,SAM
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken S- Adenosyl Methionine,SAM ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
2S)-2-Amino-4-[[(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl-methylsulfonio]butanoate; S-adenosylmethionine;
S-Adenosyl-L-methionine; SAMe; SAM;
Search name
Chicken S-Adenosyl Methionine,SAM ELISA Kit, ChickenSAM ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of S-Adenosyl Methionine,SAM concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to SAM, During the reaction, SAM in the sample or standard competes with a fixed amount of
biotin-labeled SAM for sites on a pre-coated Monoclonal antibody specific to SAM. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SAM in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验S-Adenosyl-L-Homocysteine Hydrolase
with crude rat liver extracts (Ericson et al., 1955 ). In that same year AdoHcy was chemically characterized as the product derived from S-adenosyl-L -methionine (AdoMet) via transmethylation (Baddiley and Jamieson, 1955 ), a reaction first revealed
H3 CSCH2 CH2 CH（ NH2 ） COOH，为含硫 a-氨基酸之一。是蛋白质的一种成分，卵白蛋白和酪蛋白中很多，天然得到的是 L-型。是必需氨基酸之一， L型 D型都有效。在生物体内先从 ATP接受腺苷基变成 S-腺苷酰甲硫氨酸（活性甲硫氨酸）再进行甲基转移。失去甲基的同型半胱氨酸经胱硫醚变成半胱氨酸。或直接脱去甲硫醇和氨，而间接地经同型半胱氨酸分解成α -酮酸。甲硫氨酸的生物合成是从 O-乙酰同型丝氨酸等硫化物，或由半胱氨酸的逆途径生成同型半胱氨酸（至此
藤浪肉瘤 Fujinami′s chicken sarcoma
这是鸡的肉瘤，与 Rous氏肉瘤同。此瘤由结缔组织发生，在病理学上被当作梭形细胞肉瘤。由藤浪和稻本龟五郎等（ 1912）培育肉瘤的因子已证实为藤浪肉瘤病毒（ Fujinami′ s sarcoma virus）。
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