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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine CD44 antigen,CD44
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine CD44 antigen, CD44 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CD44,SCD44,CD44 molecule; soluble CD44; cell surface glycoprotein CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; chondroitin sulfate proteoglycan 8; epican; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1, CD44 antigen,Extracellular matrix receptor III,ECMR-III,Phagocytic glycoprotein 1,PGP-1,Hyaluronate receptor,Hermes antigen,Phagocytic glycoprotein I,PGP-I,CDw44,Epican,
Search name
Canine CD44 ELISA KIT ,Canine SCD44 ELISA KIT ,Canine CD44 molecule ELISA KIT ,Canine soluble CD44 ELISA KIT ,Canine cell surface glycoprotein CD44 ELISA KIT ,Canine CDW44 ELISA KIT ,Canine CSPG8 ELISA KIT ,Canine ECMR-III ELISA KIT ,Canine HCELL ELISA KIT ,Canine HUTCH-I ELISA KIT ,Canine LHR ELISA KIT ,Canine MC56 ELISA KIT ,Canine MDU2 ELISA KIT ,Canine MDU3 ELISA KIT ,Canine MIC4 ELISA KIT ,Canine Pgp1 ELISA KIT ,Canine GP90 lymphocyte homing/adhesion receptor ELISA KIT ,Canine Hermes antigen ELISA KIT ,Canine chondroitin sulfate proteoglycan 8 ELISA KIT ,Canine epican ELISA KIT ,Canine extracellular matrix receptor III ELISA KIT ,Canine hematopoietic cell E- and L-selectin ligand ELISA KIT ,Canine heparan sulfate proteoglycan ELISA KIT ,Canine homing function and Indian blood group system ELISA KIT ,Canine hyaluronate receptor ELISA KIT ,Canine phagocytic glycoprotein 1 ELISA KIT ,Canine CD44 antigen ELISA KIT ,Canine Extracellular matrix receptor III ELISA KIT ,Canine ECMR-III ELISA KIT ,Canine Phagocytic glycoprotein 1 ELISA KIT ,Canine PGP-1 ELISA KIT ,Canine Hyaluronate receptor ELISA KIT ,Canine Hermes antigen ELISA KIT ,Canine Phagocytic glycoprotein I ELISA KIT ,Canine PGP-I ELISA KIT ,Canine CDw44 ELISA KIT ,Canine Epican ELISA KIT
Intended use
Canine CD44 ELISA KIT allows for the in vitro quantitative determination of canine CD44 antigen concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Canine CD44 ELISA KIT has been pre-coated with an antibody specific to CD44 antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD44 antigen and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CD44 antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CD44 antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Canine CD44 ELISA KIT PDF
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验CD44/CD44R 分子 CD44 常用单克隆抗体或代号: GRHL1,Hermes,F10- 44- 2(Pgp- 1H- CAM,ECM- RⅢ) 主要表达细胞: Leu,上皮,Fb,RBC[AS] 分子质量(kDa)和结构: gp80- 95 功 能: 粘附ECM,T细胞活化,淋巴细胞归位受体,归位到HEV CD44R 常用单克隆抗体或代号: FM11,24
对CD44的研究时间较长、较深入亦较广泛。已知其为分布极为广泛的细胞表面跨膜糖蛋白,主要参与异质性粘附,即肿瘤细胞与宿主细胞和宿主基质的粘附,异质性粘附在肿瘤细胞侵袭转移中起促进作用。 人类CD44基因位于11号染色体短臂,全长约50kb,共由20个高度保守的外显子组成,每个外显子长度70-210bp不等,中间由长短不一的内含子分隔。CD44基因的20个外显子按照转录方式不同可分为组成型外显子(C)和变异型拼接外显子(V)两大类。组成型外显子共有10个,存在
小鼠 CD44酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中 CD44 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 CD44水平。用纯化的 小鼠 CD44抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 CD44 ,再与HRP 标记的 CD44 抗体结合,形成抗体- 抗原 - 酶标抗体复合
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