Canine cluster of differentiat

Canine cluster of differentiation 8, CD8 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
T-cell surface glycoprotein CD8 alpha chain,; CD8A, CD8 antigen, alpha polypeptide (p32); CD8; Leu2; MAL; p32; Leu2 T-lymphocyte
antigen; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen;
CD8a molecule

Search name
Canine cluster of differentiation 8 ELISA Kit,Canine CD8 ELISA Kit, Canine T-cell surface glycoprotein CD8 alpha chain ELISA
Kit, Canine CD8A ELISA Kit, Canine CD8 antigen, alpha polypeptide (p32) ELISA Kit, Canine Leu2 ELISA Kit, Canine MAL ELISA
Kit, Canine C p32 ELISA Kit, Canine Leu2 T-lymphocyte antigen ELISA Kit, Canine OKT8 T-cell antigen ELISA Kit, Canine T cell
co-receptor ELISA Kit, Canine T-cell antigen Leu2 ELISA Kit, Canine T-lymphocyte differentiation antigen T8 ELISA Kit, Canine
Leu-2 ELISA Kit, Canine T8 T-cell antigen ELISA Kit, Canine CD8a molecule ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine T-cell surface glycoprotein CD8 alpha chain
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-cell surface glycoprotein CD8 alpha chain.
Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation
specific for T-cell surface glycoprotein CD8 alpha chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain T-cell surface
glycoprotein CD8 alpha chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T-cell surface glycoprotein CD8 alpha chain in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF
 

  "(本产品仅供体外科研用途，不可用于临床诊断!)