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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Chicken Leptin
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Leptin ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Leptin,Obesity factor, OBS,obese protein,obese,
Search name
Chicken Leptin ELISA Kit,Leptin ELISA Kit,
Leptin ELISA KIT,Obesity factor ELISA KIT, OBS ELISA KIT,obese protein ELISA KIT,obese ELISA KIT,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of chicken Leptin concentrations in serum, Plasma, Urine, tissue
homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Leptin and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Leptin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Leptin in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 猪瘦素 ( Leptin )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗猪 Leptin 单抗包被于酶标板上,标准品和样品中的 Leptin与单抗结合,加入生物素化的抗猪
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 狗瘦素 (Leptin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗狗 Leptin 单抗包被于酶标板上,标准品和样品中的 Leptin 与单抗结合,加入生物素化的抗狗 Leptin ,形成免疫复合物连接在板上,辣根
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人瘦素 (Leptin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Leptin 单抗包被于酶标板上,标准品和样品中的 Leptin与单抗结合,加入生物素化的抗人
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