- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-13883d
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine MX1
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine MX1 ELISA KIT
Product Name:Canine MX1 ELISA KIT
Packing:96T
Catalog No.:ELI-13883d
Gene Name:Canine MX1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine MX1
Alternative Name:MX1,MX1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine MX1 ELISA KIT allows for the in vitro quantitative determination of Canine MX1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine MX1 ELISA KIT has been pre-coated with an Canine MX1 antibody specific to Canine MX1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine MX1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine MX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine MX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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是很高。该基因在同一种生物的不同品种中具有多态性，已经有其它的鸡的Mx基因被克隆，按道理如果该片段是Mx基因的一部分它应该与其它的鸡的Mx基因有很高的同源性才对呀，所以，我怀疑简并引物PCR扩增出来的该片段不是Mx基因，因为RACE试剂盒很贵，现在也不敢继续作下去，怕作下去得不到什么结果。现在贴上我BLAST结果中score排在最前面的部分，还请有经验的兄弟帮我分析分析。我不胜感激。 Sequences producing significant alignments: (bits
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