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Canine Type I iodothyronine de

iodinase,DIO1 ELISA试剂盒
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  • ¥3480
  • LSM BIO
  • 0.78-50ng/ml
  • 0.469ng/ml
  • 进口
  • ELI-39333d
  • 2025年07月13日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      100

    • 供应商

      LSM BIO

    • 检测范围

      0.78-50ng/ml

    • 检测方法

      夹心法

    • 应用

      利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度

    • 适应物种

    • 标记物

      Canine Type I iodothyronine deiodinase,DIO1

    • 样本

      狗的血浆,血清,细胞上清等

    • 灵敏度

      0.469ng/ml

    • 规格

      96tests

    Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT

    Product Name:Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT
    Packing:96T

    Catalog No.:ELI-39333d

    Gene Name:Canine Type I iodothyronine deiodinase, DIO1

    Detect Range:31.25-2000pg/ml

    Sensitivity:18.75pg/ml

    Target Protein Name:Canine Type I iodothyronine deiodinase, DIO1

    Alternative Name:Type I iodothyronine deiodinase,Type I iodothyronine deiodinase, DIO1
    Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA type:Sandwich ELISA Kit
    Product Description:Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT allows for the in vitro quantitative determination of Canine Type I iodothyronine deiodinase, DIO1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA Test Principle:
    The microtiter plate provided in

    Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT

    96 Tests

    Operating instructions

     

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

     

    Synonyms

    DIO1,5DI; TXDI1; DIOI; thyroxine deiodinase type I selenoprotein; type 1 DI; type I iodothyronine deiodinase; type-I 5'-deiodinase; deiodinase iodothyronine, type I ,Type I iodothyronine deiodinase,Type-I 5'-deiodinase,Type 1 DI,5DI,DIOI,

     

    Search name

    Canine DIO1 ELISA KIT ,Canine 5DI ELISA KIT ,Canine TXDI1 ELISA KIT ,Canine DIOI ELISA KIT ,Canine thyroxine deiodinase type I selenoprotein ELISA KIT ,Canine type 1 DI ELISA KIT ,Canine type I iodothyronine deiodinase ELISA KIT ,Canine type-I 5'-deiodinase ELISA KIT ,Canine deiodinase iodothyronine type I ELISA KIT ,Canine Type I iodothyronine deiodinase ELISA KIT ,Canine Type-I 5'-deiodinase ELISA KIT ,Canine Type 1 DI ELISA KIT ,Canine 5DI ELISA KIT ,Canine DIOI ELISA KIT

     

    Intended use

    This immunoassay kit allows for the in vitro quantitative determination of Canine type Type I iodothyronine deiodinase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

     

    Test principle

    The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Type I iodothyronine deiodinase, During the reaction, Type I iodothyronine deiodinase in the sample or standard competes with a fixed amount of biotin-labeled Type I iodothyronine deiodinase for sites on a pre-coated Monoclonal antibody specific to Type I iodothyronine deiodinase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Type I iodothyronine deiodinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    has been pre-coated with an Canine Type I iodothyronine deiodinase, DIO1 antibody specific to Canine Type I iodothyronine deiodinase, DIO1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Type I iodothyronine deiodinase, DIO1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Type I iodothyronine deiodinase, DIO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Type I iodothyronine deiodinase, DIO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

      "(本产品仅供体外科研用途,不可用于临床诊断!)

     

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    图标文献和实验
    该产品被引用文献
    Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstrm1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3* First Published on Journal of biological chemistry September 19, 2019 doi: 10.1074/jbc.RA119.010483 jbc.RA119.010483.
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      Bassett, J.H., Boyde, A., Howell, P.G., Bassett, R.H., Galliford, T.M., Archanco, M., Evans, H., Lawson, M.A., Croucher, P., St Germain, D.L., Galton, V.A., and Williams, G.R. 2010. Optimal bone strength and mineralization requires the type 2 iodothyronine

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    Canine Type I iodothyronine deiodinase,DIO1 ELISA试剂盒
    ¥3480