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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Retinoid isomerohydrolase,RPE65
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Retinoid isomerohydrolase, RPE65 ELISA KIT
Product Name:Canine Retinoid isomerohydrolase, RPE65 ELISA KIT
Packing:96T
Catalog No.:ELI-38680d
Gene Name:Canine Retinoid isomerohydrolase, RPE65
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine Retinoid isomerohydrolase, RPE65
Alternative Name:Retinoid isomerohydrolase,Retinoid isomerohydrolase, RPE65
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Retinoid isomerohydrolase, RPE65 ELISA KIT allows for the in vitro quantitative determination of Canine Retinoid isomerohydrolase, RPE65 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Retinoid isomerohydrolase, RPE65 ELISA KIT 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms RPE65,BCO3; LCA2; RP20; mRPE65; rd12; sRPE65; Retinoid isomerohydrolase,BCO family member 3; RBP-binding membrane protein; all-trans-retinyl-palmitate hydrolase; p63; retinal pigment epithelium-specific 65 kDa protein; retinitis pigmentosa 20 autosomal recessive; retinol isomerase; retinal pigment epithelium-specific protein 65kDa Search name Canine RPE65 ELISA KIT ,Canine BCO3 ELISA KIT ,Canine LCA2 ELISA KIT ,Canine RP20 ELISA KIT ,Canine mRPE65 ELISA KIT ,Canine rd12 ELISA KIT ,Canine sRPE65 ELISA KIT ,Canine Retinoid isomerohydrolase ELISA KIT ,Canine BCO family member 3 ELISA KIT ,Canine RBP-binding membrane protein ELISA KIT ,Canine all-trans-retinyl-palmitate hydrolase ELISA KIT ,Canine p63 ELISA KIT ,Canine retinal pigment epithelium-specific 65 kDa protein ELISA KIT ,Canine retinitis pigmentosa 20 autosomal recessive ELISA KIT ,Canine retinol isomerase ELISA KIT ,Canine retinal pigment epithelium-specific protein 65kDa ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of canine RPE65 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPE65. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RPE65 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RPE65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RPE65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina
- retinyl ester (all-trans- RE) or an all-trans- retinol (all-trans- ROL). In RPE cells, this step is carried out by an enzyme called Rpe65 isomerase. This chapter provides methods for assaying Rpe65 isomerase. Although Rpe65 utilizes
Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle
and retinoid content of the retina can provide important insights into the mechanisms of disease initiation and progression. Here, we use the protein RPE65 as an example to describe generally applicable methods for determining
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