- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-29024d
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine Tudor domain-containing protein 5,TDRD5
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine Tudor domain-containing protein 5, TDRD5 ELISA KIT
Product Name:Canine Tudor domain-containing protein 5, TDRD5 ELISA KIT
Packing:96T
Catalog No.:ELI-29024d
Gene Name:Canine Tudor domain-containing protein 5, TDRD5
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Canine Tudor domain-containing protein 5, TDRD5
Alternative Name:Tudor domain-containing protein 5,Tudor domain-containing protein 5, TDRD5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Tudor domain-containing protein 5, TDRD5 ELISA KIT allows for the in vitro quantitative determination of Canine Tudor domain-containing protein 5, TDRD5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Tudor domain- containing protein 5, TDRD5 ELISA Kit 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms TDRD5,TUDOR3; tudor domain-containing protein 5; tudor domain containing 5 Search name Canine TDRD5 ELISA KIT ,Canine TUDOR3 ELISA KIT ,Canine tudor domain-containing protein 5 ELISA KIT ,Canine tudor domain containing 5 ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of Canine Tudor domain- containing protein 5, TDRD5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to TDRD5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TDRD5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TDRD5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TDRD5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验Protein Carbonyl Measurement by ELISA
) (2,3). Protein-bound DNP can also be measured with increased sensitivity by enzyme-linked immunosorbent assay (ELISA) (4,5). Samples containing protein are reacted with DNP, then the protein is nonspecifically adsorbed to an ELISA plate. Unconjugated DNP
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
Figure 17.4.1 Photograph of a phage ELISA result. Using eight different clones that were isolated with GST‐SrcSH3 domain as the target, 25 µl of culture supernatant were added to pairs of microtiter plate wells containing GST‐SrcSH3 or GST
Protein A Purification of Antibody
fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L
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