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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Mitochondrial uncoupling protein 2,UCP2
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Mitochondrial uncoupling protein 2, UCP2 ELISA KIT
Product Name:Canine Mitochondrial uncoupling protein 2, UCP2 ELISA KIT
Packing:96T
Catalog No.:ELI-06626d
Gene Name:Canine Mitochondrial uncoupling protein 2, UCP2
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Canine Mitochondrial uncoupling protein 2, UCP2
Alternative Name:Mitochondrial uncoupling protein 2,Mitochondrial uncoupling protein 2, UCP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Mitochondrial uncoupling protein 2, UCP2 ELISA KIT allows for the in vitro quantitative determination of Canine Mitochondrial uncoupling protein 2, UCP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Mitochondrial uncoupling protein 2,UCP 2 ELISA Kit 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms UCP2,uncoupling protein 2,BMIQ4; SLC25A8; UCPH; solute carrier family 25 member 8; uncoupling protein 2 Search name Canine UCP2 ELISA KIT ,Canine uncoupling protein 2 ELISA KIT ,Canine BMIQ4 ELISA KIT ,Canine SLC25A8 ELISA KIT ,Canine UCPH ELISA KIT ,Canine solute carrier family 25 member 8 ELISA KIT ,Canine uncoupling protein 2 ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of canine mitochondrial uncoupling protein 2,UCP 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCP 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for UCP 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UCP 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UCP 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in �regulating apoptosis in different cell systems
, mitochondrial uncoupling proteins (UCPs) displayed (non-native) artefactual uncoupling when expressed in yeast or can possess functions upon reconstitution in proteoliposomes that cannot be reproduced in isolated mitochondria. In the light of newly discovered
Cell Metab:北大邱义福团队发现「产热通道」介导的褐色脂肪产热新机制
肥胖已成为全球性的重大健康问题,在个体层面可以通过增加能量消耗来对抗肥胖。自 2009 年在成人中检测到代谢活跃的褐色/米色脂肪组织(BAT (brown adipose tissue) or beige fat)以来,科学家们一直在寻找增强褐色/米色脂肪产热从而增加能量消耗、抵抗肥胖的方法。 褐色脂肪组织线粒体富含解偶联蛋白 UCP1(uncoupling protein 1)。UCP1 能够提高线粒体内膜对质子的电导性,从而消耗质子梯度,将氧化磷酸化与 ATP 合成解偶联,把底物氧
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