Canine Corticoliberin,CRH ELIS

Canine Corticoliberin, CRH ELISA KIT

Product Name:Canine Corticoliberin, CRH ELISA KIT
Packing:96T

Catalog No.:ELI-02826d

Gene Name:Canine Corticoliberin, CRH

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine Corticoliberin, CRH

Alternative Name:Corticoliberin,Corticoliberin, CRH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Corticoliberin, CRH ELISA KIT allows for the in vitro quantitative determination of Canine Corticoliberin, CRH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Corticoliberin, CRH ELISA KIT
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Corticoliberin,Corticotropin-releasing factor(CRF),Corticotropin-releasing hormone, CRH,corticotropin-releasing factor; CRF;
corticoliberin; corticotropin releasing hormone

Search name
Canine Corticoliberin ELISA Kit, Canine CRF ELISA Kit, Canine corticotropin releasing hormone ELISA Kit, Canine CRH ELISA
Kit, Corticoliberin ELISA Kit, CRF ELISA Kit, corticotropin releasing hormone ELISA Kit, CRH ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine corticoliberin,Corticoliberin concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Corticoliberin, During the reaction, Corticoliberin in the sample or standard competes with a fixed
amount of biotin-labeled Corticoliberin for sites on a pre-coated Monoclonal antibody specific to Corticoliberin. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Corticoliberin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Corticoliberin, CRH antibody specific to Canine Corticoliberin, CRH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Corticoliberin, CRH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Corticoliberin, CRH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Corticoliberin, CRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

  "(本产品仅供体外科研用途，不可用于临床诊断!)