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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Heat shock protein beta-1,HSPB1
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Heat shock protein beta-1, HSPB1 ELISA KIT
Product Name:Canine Heat shock protein beta-1, HSPB1 ELISA KIT
Packing:96T
Catalog No.:ELI-02413d
Gene Name:Canine Heat shock protein beta-1, HSPB1
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine Heat shock protein beta-1, HSPB1
Alternative Name:Heat shock protein beta-1,Heat shock protein beta-1, HSPB1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Heat shock protein beta-1, HSPB1 ELISA KIT allows for the in vitro quantitative determination of Canine Heat shock protein beta-1, HSPB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Heat shock protein beta- 1, HSPB1 ELISA KIT FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE Synonyms Search name Intended use Test principle
The microtiter plate provided in
96 Tests
Operating instruction
BEGINNING!
Heat shock protein beta-1(HspB1),Heat shock 27 kDa protein(HSP 27),; HSP 27; CMT2F; HMN2B; HS.76067; HSP28; Hsp25; SRP27;
28 kDa heat shock protein; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock protein
beta-1; stress-responsive protein 27; heat shock 27kDa protein 1,
Canine Heat shock protein beta-1 ELISA Kit, Canine HspB1 ELISA Kit, Canine Heat shock 27 kDa protein ELISA Kit, Canine HSP
27 ELISA Kit, Canine CMT2F ELISA Kit, Canine HMN2B ELISA Kit, Canine HS.76067 ELISA Kit, Canine HSP28 ELISA Kit, Canine
Hsp25 ELISA Kit, Canine SRP27 ELISA Kit, Canine stress-responsive protein 27 ELISA Kit, Canine Heat shock 28 kDa protein
ELISA Kit, Canine Heat shock 25 kDa protein ELISA Kit, Canine estrogen-regulated 24 kDa protein ELISA Kit,
This immunoassay kit allows for the in vitro quantitative determination of Canine heat shock protein beta-1,HspB1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HspB1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HspB1 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain HspB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HspB1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Isolation of Heat Shock Protein Complexes
Heat shock proteins (Hsp) are molecular chaperones with the capability to interact with a wide range of other proteins and are thus often found coupled with other heat shock and non-heat shock proteins. This can be an advantage to study
指将生物的整体、组织、细胞等从其生活的温度范围内急剧地从低温移向高温时,可显著地促进合成的一组蛋白质。例如将果蝇的幼虫或培养细胞从28℃移至 35℃时,则几乎大部分的蛋白质合成停止;与此相反,而休克蛋白的合成却反而被促进。这种促进作用主要是在转录 DNA的合成(转录)阶段产生的。同样的现象也见于哺乳类动物、培养细胞、原生动物、植物组织和细菌等。另外观察到,由休克以外的其他处理也会发生类似的现象。这种现象的生理意义尚不清楚,但推测是与生物的温度适应现象有关系。
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
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