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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine TMEM47
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine TMEM47 ELISA KIT
Product Name:Canine TMEM47 ELISA KIT
Packing:96T
Catalog No.:ELI-41880d
Gene Name:Canine TMEM47
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine TMEM47
Alternative Name:Transmembrane protein 47,TMEM47
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine TMEM47 ELISA KIT allows for the in vitro quantitative determination of Canine TMEM47 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine TMEM47 ELISA KIT has been pre-coated with an Canine TMEM47 antibody specific to Canine TMEM47 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine TMEM47 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine TMEM47, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine TMEM47 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验>>> 分装 ELISA 试剂盒 F28100 狗脂联素 (Canine adiponectin) ELISA 48T/96T 2000/3000 进口分装 F28110 狗 C
的,因此检验人员需经过培训,熟练掌握本专业如下几方面的技术知识:◎检验项目的基本原理 (ELISA原理);◎临床意义;◎熟悉检测技巧,了解易出差错的环节及难点;◎熟悉检测试剂性能(包括试剂盒组成,包被片段及其组成);◎ 熟悉检测仪器的原理及性能;掌握数据处理的能力和质量控制知识。◎某些特殊项目的检测如抗HIV等需经有关部门组织的专门培训班,考试合格后持证上岗。◎ELISA原理:1971年Engvall等人把免疫组化的EIA技术应用于临床检验发展为ELISA技术。 特点 :在固相表面组装免疫活性物质形成
检验项目的基本原理 (ELISA原理); ◎临床意义; ◎熟悉检测技巧,了解易出差错的环节及难点; ◎熟悉检测试剂性能(包括试剂盒组成,包被片段及其组成); ◎ 熟悉检测仪器的原理及性能;掌握数据处理的能力和质量控制知识。 ◎某些特殊项目的检测如抗HIV等需经有关部门组织的专门培训班,考试合格后持证上岗。 分析前质控 ◎ELISA原理:1971年Engvall等人把免疫组化的EIA技术应用于临床检验发展为ELISA技术。 特点 :在固相表面组装免疫活性物质形成"免疫
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