- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-41321d
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine RHOA
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine RHOA ELISA KIT
Product Name:Canine RHOA ELISA KIT
Packing:96T
Catalog No.:ELI-41321d
Gene Name:Canine RHOA
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine RHOA
Alternative Name:Transforming protein RhoA,RHOA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine RHOA ELISA KIT allows for the in vitro quantitative determination of Canine RHOA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine RHOA ELISA KIT has been pre-coated with an Canine RHOA antibody specific to Canine RHOA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine RHOA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine RHOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine RHOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验莲芯 请各位高人指点，在RhoA-ROCK信号通路中，如何用实验解析RhoA是怎么活化的呢,并且ROCk是如何参与细胞运动的．十分感谢！急呀！ dianaofyezi 可以购买相应的试剂盒进行检测活化后的RHO-A蛋白。 doctormy "如何用实验解析RhoA是怎么活化的呢"，如果我没理解错的话，你的意思是解释和分析，这是试验设计的基本问题，只有结合你的课题具体思路去设计解释。
用RhoA抗体检测沉淀物中RhoA-GTP含量。试剂盒是upstate及cytoskeleton公司都有。但是区别upstate测的是活性Rho，而细胞骨架公司的测的是活化的RhoA。看了说明书，操作中要液氮，要低温，要保持RhoA的活性不被降解，而且试剂盒贵，1万元左右。 4、看了还有G-LISA的方法，用抗体与活化的RHOA结合的原理。用的是抗GTP-RhoA。 有人做过相关的检测吗？这几种方法有缺点，或者说更科学合理的道理在何处？求解，谢谢
>>> 分装 ELISA 试剂盒 F28100 狗脂联素 (Canine adiponectin) ELISA 48T/96T 2000/3000 进口分装 F28110 狗 C
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