- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03095d
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine APP
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine APP ELISA KIT
Product Name:Canine APP ELISA KIT
Packing:96T
Catalog No.:ELI-03095d
Gene Name:Canine APP
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine APP
Alternative Name:Amyloid beta A4 protein,APP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine APP ELISA KIT allows for the in vitro quantitative determination of Canine APP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine APP ELISA KIT has been pre-coated with an Canine APP antibody specific to Canine APP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine APP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine APP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine APP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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at 405nm. For HRP conjugates: Mix equal parts of the TMB system, place in wells, allow to develop until desired intensity (app. 5 min) and stop reaction by adding 100 µl of 1M Phosphoric Acid. Read at 450 nm. Conjugate Preparation 1. Centrifuge
Quantifying Aβ1–40 and Aβ1–42 Using Sandwich-ELISA
The role of Aβ accumulation in the pathogenesis of Alzheimer’s disease (AD) is supported by genetic studies showing that mutations in the amyloid-β precursor protein (APP) that alter Aβ production are linked to a subset of familial AD (FAD) cases
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