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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Chicken IgE
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken IgE ELISA Kit
Product Name:Chicken IgE ELISA Kit
Packing:96T
Catalog No.:ELA-E0545d
Gene Name:Chicken IgE
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Chicken IgE
Alternative Name:Immunoglobulin E,IgE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Chicken IgE ELISA Kit allows for the in vitro quantitative determination of Chicken IgE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Chicken IgE ELISA Kit has been pre-coated with an Chicken IgE antibody specific to Chicken IgE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Chicken IgE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Chicken IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Chicken IgE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验大鼠卵清蛋白IgE(OVA IgE)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠卵清蛋白 IgE(OVA IgE)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 OVA IgE 单抗包被于酶标板上,标准品和样品中的 OVA IgE 与单抗结合,加入生物素化的抗大鼠 OVA IgE ,形成免疫
Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
大鼠 免疫球蛋白 E(IgE) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中 免疫球蛋白E(IgE) 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 大鼠 免疫球蛋白 E(IgE) 水平。用纯化的大鼠 IgE 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 免疫球蛋白 E(IgE
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