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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Inositol trisphosphate,InsP3
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Inositol trisphosphate, InsP3 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Inositol trisphosphate,Inositol 1,4,5-trisphosphate, triphosphoinositol,InsP3,Ins3P,IP3
Search name
Canine Inositol trisphosphate ELISA KIT ,Canine Inositol 1, 4, 5-trisphosphate ELISA KIT ,Canine triphosphoinositol ELISA KIT ,Canine InsP3 ELISA KIT ,Canine Ins3P ELISA KIT ,Canine IP3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Inositol trisphosphate concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol trisphosphate, During the reaction, Inositol trisphosphate in the sample or standard competes with a fixed amount of biotin-labeled Inositol trisphosphate for sites on a pre-coated Monoclonal antibody specific to Inositol trisphosphate. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inositol trisphosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Inositol 1,4,5-Trisphosphate Mass Assay
-4,5-bisphosphate (PtdInsP2 ) and generates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3 ), which in turn releases calcium from intracellular stores (1 ).
Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
Inositol(1,4,5)trisphosphate [Ins(1,4,5)P3 ] is an important second messenger that activates its cognate Ins(1,4,5)P3 receptor to release Ca2+ from intracellular stores. The assay described in this chapter uses the Ins(1,4,5)P3 receptor
Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle
Calcium transients elicited by IP3 receptors upon electrical stimulation of skeletal muscle cells (slow calcium signals) are often hard to visualize due to their relatively small amplitude compared to the large transient originated
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