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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Phosphatidylserine,PS
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Phosphatidylserine, PS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Phosphatidylserine, Ptd-L-Ser ,PS
Search name
Canine Phosphatidylserine ELISA Kit, Canine PS ELISA Kit, Canine Ptd-L-Ser ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Phosphatidylserine concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Phosphatidylserine, During the reaction, Phosphatidylserine in the sample or standard competes
with a fixed amount of biotin-labeled Phosphatidylserine for sites on a pre-coated Monoclonal antibody specific to Phosphatidylserine.
Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)
is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Phosphatidylserine in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Q:Annexin V 凋亡检测试剂盒的原理是什么? A:Annexin V 是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸 PS 有高度亲和力,可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合,将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,核染料 PI 或 7-AAD
【心得】关于全血的保存-DNA提取-pcr个人经验(经常在线回复)(血中rna提取在后边ps:5)(组织中提DNA ps6)
500ul加100溶解液Ps5:提取血中RNA可以先提取淋巴细胞在,然后再从淋巴细胞里边提取RNA-按一般的trizol方法即可,直接用血和trizol效果不好-我做的时候是这样的ps6:离心柱法提取血的试剂盒来提取组织,呵呵省的买专门提取组织的了省点钱!!!称量小于50-20mg的组织(20一般够了)与EP管中,(括号内的可以省略,加了之后效果好产率高不少:打碎处理为细胞悬液,然后10,000rpm(~11,200×g)离心1 min,倒尽上清,,也可以稍微剪成小块这样倒是不复杂)加入200?l
>>> 分装 ELISA 试剂盒 F28100 狗脂联素 (Canine adiponectin) ELISA 48T/96T 2000/3000 进口分装 F28110 狗 C
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