- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66009d
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine Fructosamine,FA
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine Fructosamine, FA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Canine Fructosamine ELISA Kit, Canine FA ELISA Kit,Fructosamine ELISA Kit,FA ELISA Kit,ELISA Kit, Fructosamine,FA
Intended use
Canine Fructosamine ELISA Kit allows for the in vitro quantitative determination of Canine fructosamine,FA concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Fructosamine, During the reaction, Fructosamine in the sample or standard competes with a fixed
amount of biotin-labeled Fructosamine for sites on a pre-coated Monoclonal antibody specific to Fructosamine. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Fructosamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 15.6-1000 umol/l
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离心5分钟1.6.将上清倒去，使用FA裂解液将沉淀重悬浮（1x107cells/750μl).初始细胞要有1*107-5*107个，采用终浓度为0.75%甲醛和如上描述的甘氨酸处理。预冷PBS洗3次，1，000g离心5分钟，沉淀用FA裂解液重悬浮。2 超声破碎超声裂解细胞悬液可以将DNA均一的打断成500-1000bp的片段。不同的细胞系需要不同的超声时间才能达到最优效果。交联细胞要通过时间梯度的超声来选择最优超声条件。样品通过时间梯度，DNA的分离如部分3所描述。片段大小序在1.5%的琼脂
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