- ￥2260
- LSM Bio
- 进口
- PAab09948
- 2025年07月15日
- ELISA, WB, IF
- E. coli - derived recombinant protein
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Phospho-CREB1-S133
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
polyclonal
- 标记物：
Non-conjugated
- 适应物种：
Human
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, WB, IF
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
Phospho-CREB1-S133
- 抗体英文名：
Phospho- CREB1(S133) antibody
- 抗体名：
Phospho- CREB1(S133) antibody
- 规格：
100μl
Phospho- CREB1(S133) antibody
Product Name Phospho- CREB1(S133) antibody
Catalog No PAab09948
Packing 100ul
Form liquid
Alternative Name CREB, CREB-1
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Immunogen Phospho-CREB1-S133
Specificity Human
Tested Application ELISA, WB, IF
Recommended dilution WB: 1:500 - 1:2000; IF: 1:50 - 1:200
IHC use
WB use
293T cells were subjected to SDS PAGE followed by western blot with PAab09948(Phospho- CREB1(S133) antibody) at dilution of 1:1000. 293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Protein Information
Gene ID 1385
Uniprot ID P16220
Calculated MW 42kDa
Research Area Signal Transduction, Metabolism, Cardiovascular, Immunology, Cancer, Developmental biology, Neuroscience
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文献和实验Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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