- ￥2260
- LSM Bio
- 进口
- PAab09943
- 2025年07月14日
- ELISA, WB, IHC
- E. coli - derived recombinant protein
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic phosphorylated peptide around Thr246 of human AKT1S1
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
polyclonal
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, WB, IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
A synthetic phosphorylated peptide around Thr246 of human AKT1S1
- 抗体英文名：
Phospho-AKT1S1(T246) antibody
- 抗体名：
Phospho-AKT1S1(T246) antibody
- 规格：
100μl
Phospho-AKT1S1(T246) antibody
Product Name Phospho-AKT1S1(T246) antibody
Catalog No PAab09943
Packing 100ul
Form liquid
Alternative Name AKT1S1, Lobe, PRAS40, AKT1 substrate 1
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Immunogen A synthetic phosphorylated peptide around Thr246 of human AKT1S1
Specificity Human, Mouse, Rat
Tested Application ELISA, WB, IHC
Recommended dilution WB: 1:500 - 1:2000; IHC: 1:20 - 1:200
IHC use Immunohistochemistry of paraffin-embedded rat liver tissue slide using PAab09943(Phospho-AKT1S1(T246) Antibody) at dilution of 1:100
WB use
Hela cells were subjected to SDS PAGE followed by western blot with PAab09943(Phospho-AKT1S1(T246) Antibody) at dilution of 1:1000,HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight.
Protein Information
Gene ID
Uniprot ID Q96B36
Calculated MW 50 kDa
Research Area
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文献和实验Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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