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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
peptide
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9BZJ3
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA, WB
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
peptide
- 抗体英文名:
Phospho-BTK(Tyr 223) Antibody
- 抗体名:
Phospho-BTK(Tyr 223) Antibody
- 规格:
100μl
Phospho-BTK(Tyr 223) Antibody
Product Name Phospho-BTK(Tyr 223) Antibody
Catalog No PAab09846
Packing 100ul
Form liquid
Alternative Name Agammaglobulinemia tyrosine kinase,B-cell progenitor kinase,Bruton tyrosine kinase,ATK,BPK,AGMX1
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Defects in the Bruton tyrosine kinase(BTK) gene cause Agammaglobulinemia. Agammaglobulinemia is an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement.
Immunogen peptide
Specificity Human, Mouse, Rat
Tested Application ELISA, WB
Recommended dilution WB: 1:500-1:2000
IHC use
WB use
Hela cell lysate were subjected to SDS PAGE followed by western blot with PAab09846(Phospho-BTK(Tyr223) Antibody) at dilution of 1:1000
Protein Information
Gene ID
Uniprot ID Q06187
Calculated MW 52 kDa
Research Area
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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