- ￥2260
- LSM Bio
- 进口
- PAab10100
- 2025年07月16日
- ELISA, IHC
- E. coli - derived recombinant protein
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
O-6-methylguanine-DNA methyltransferase
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
polyclonal
- 标记物：
Non-conjugated
- 适应物种：
Human
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
O-6-methylguanine-DNA methyltransferase
- 抗体英文名：
MGMT antibody
- 抗体名：
MGMT antibody
- 规格：
100μl
MGMT antibody
Product Name MGMT antibody
Catalog No PAab10100
Packing 100ul
Form liquid
Alternative Name MGMT
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Involved in the cellular defense against the biological effects of O6-methylguanine(O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Immunogen O-6-methylguanine-DNA methyltransferase
Specificity Human
Tested Application ELISA, IHC
Recommended dilution IHC: 1:50 - 1:500
IHC use Immunohistochemistry of paraffin-embedded human liver cancer using PAab10100(MGMT antibody) at dilution of 1:200
WB use
Protein Information
Gene ID 4255
Uniprot ID P16455
Calculated MW 22 kDa
Research Area Cancer, Immunology, Metabolism
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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