- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-52840b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Ribonuclease P protein subunit p30,RPP30
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Ribonuclease P protein subunit p30, RPP30 ELISA KIT
Product Name:Bovine Ribonuclease P protein subunit p30, RPP30 ELISA KIT
Packing:96T
Catalog No.:ELI-52840b
Gene Name:Bovine Ribonuclease P protein subunit p30, RPP30
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Ribonuclease P protein subunit p30, RPP30
Alternative Name:RPP30,Bovine Ribonuclease P protein subunit p30, RPP30
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Ribonuclease P protein subunit p30, RPP30 ELISA KIT allows for the in vitro quantitative determination of Bovine Ribonuclease P protein subunit p30, RPP30 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Ribonuclease P protein subunit p30, RPP30 ELISA KIT has been pre-coated with an Bovine Ribonuclease P protein subunit p30, RPP30 antibody specific to Bovine Ribonuclease P protein subunit p30, RPP30 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Ribonuclease P protein subunit p30, RPP30 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Ribonuclease P protein subunit p30, RPP30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Ribonuclease P protein subunit p30, RPP30 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Ribonuclease P (RNase P) from Escherichia coli is a transfer RNA (tRNA)-processing enzyme and consists of a catalytic RNA subunit (M1 RNA) and a protein component (C5 protein). M1GS, a gene-targeting ribozyme derived from M1 RNA, can cleave
subunits homologous to eukaryotic nuclear RNase P proteins. RNA. 2002, 8 (3): 296-306. PMID 12003490. ^ (英文)Fukuhara H, Kifusa M, Watanabe M, Terada A, Honda T, Numata T, Kakuta Y, Kimura M. A fifth protein subunit Ph1496p elevates the optimum
to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
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