- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0104b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine 5-Nucleotidase,5-NT
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine 5- Nucleotidase, 5- NT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NT5E,CALJA; E5NT; NT5; NTE; eNT; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; 5'-nucleotidase ecto, 5'-nucleotidase,
Search name
Bovine NT5E ELISA KIT ,Bovine CALJA ELISA KIT ,Bovine E5NT ELISA KIT ,Bovine NT5 ELISA KIT ,Bovine NTE ELISA KIT ,Bovine eNT ELISA KIT ,Bovine 5'-NT ELISA KIT ,Bovine Purine 5-Prime-Nucleotidase ELISA KIT ,Bovine ecto-5'-nucleotidase ELISA KIT ,Bovine 5'-nucleotidase ecto ELISA KIT ,Bovine 5'-nucleotidase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine 5'-nucleotidase,5'-NT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-NT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 5'-NT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 5'-NT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5'-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
大鼠 5- 核苷酸酶 (5-NT)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 5-NT 单抗包被于酶标板上，标准品和样品中的 5-NT 与单抗结合，加入生物素化的抗大鼠 5-NT ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm
TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
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