- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0460b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Dehydroepiandrosterone sulfate,DHEAS
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Dehydroepiandrosterone sulfate, DHEAS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Dehydroepiandrosterone sulfate,DHEA sulfate,DHEA-ST,DHEAS,HST,ST2,ST2A1,ST2A3,STD,hSTa,alcohol/hydroxysteroid
sulfotransferase,bile salt sulfotransferase,bile-salt sulfotranasferase 2A1, SULT2A1
Search name
Bovine Dehydroepiandrosterone sulfate ELISA Kit, Bovine DHEAS ELISA Kit,Dehydroepiandrosterone sulfate ELISA Kit,DHEAS
ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Dehydroepiandrosterone sulfate,DHEA sulfate
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DHEA sulfate. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DHEA sulfate and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain DHEA sulfate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA sulfate in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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文献和实验4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Reagents Ammonium sulfate (50% [w/v]) (optional, see Step 23) Antibodies, primary Affinity-purified primary antibodies are diluted in PBS containing 1% BSA. The dilutions must be adjusted for each individual primary antibody. Typical
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