- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0723b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Chondroitin sulfate,CS
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Chondroitin sulfate ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Chondroitin sulfate,;
Search name
Bovine Chondroitin sulfate ELISA Kit, Bovine CS ELISA Kit, Chondroitin sulfate ELISA Kit, CS ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of chondroitin sulfate,Chondroitin sulfate concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Chondroitin sulfate, During the reaction, Chondroitin sulfate in the sample or standard competes
with a fixed amount of biotin-labeled Chondroitin sulfate for sites on a pre-coated Monoclonal antibody specific to Chondroitin sulfate.
Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)
is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Chondroitin sulfate in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
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文献和实验Procedures for In Vitro Production of Bovine Embryos
Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed
4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates
head is not defined. 9. A minimum of 100 sperm should be count to determine the percentages of acrosome membrane integrity. Reference R.W. Lenz, G.D. Ball, J.K. Lohse, N.L. First and R.L. Ax. Chondroitin sulfate facilitates
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