Bovine Vitamin D2,VD2 ELISA Ki

Bovine Vitamin D2, VD2 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vitamin D2,VD2

Search name
Bovine Vitamin D2 ELISA Kit, Bovine VD2 ELISA Kit, Vitamin D2 ELISA Kit, VD2 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general Vitamin D2,VD2 concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to VD2, During the reaction, VD2 in the sample or standard competes with a fixed amount of
biotin-labeled VD2 for sites on a pre-coated Monoclonal antibody specific to VD2. Excess conjugate and unbound sample or standard are
washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a
TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VD2 in the samples is then
determined by comparing the O.D. of the samples to the standard curve.