- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0162b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Glutamine synthetase,GS
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Glutamine synthetase,GS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutamine synthetase,GS,Glutamate decarboxylase,Glutamate--ammonia ligase,Glutamine synthetase,GLNS; PIG43; PIG59; cell proliferation-inducing protein 59; glutamate decarboxylase; glutamate--ammonia ligase; glutamine synthase; proliferation-inducing protein 43; glutamate-ammonia ligase,GLUL
Search name
Bovine Glutamine synthetase ELISA KIT ,Bovine GS ELISA KIT ,Bovine Glutamate decarboxylase ELISA KIT ,Bovine Glutamate--ammonia ligase ELISA KIT ,Bovine Glutamine synthetase ELISA KIT ,Bovine GLNS ELISA KIT ,Bovine PIG43 ELISA KIT ,Bovine PIG59 ELISA KIT ,Bovine cell proliferation-inducing protein 59 ELISA KIT ,Bovine glutamate decarboxylase ELISA KIT ,Bovine glutamate--ammonia ligase ELISA KIT ,Bovine glutamine synthase ELISA KIT ,Bovine proliferation-inducing protein 43 ELISA KIT ,Bovine glutamate-ammonia ligase ELISA KIT,Bovine GLUL ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine glutamine synthetase,GS concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Procedures for In Vitro Production of Bovine Embryos
Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed
expression studiesAvailable in the following LABORATORY:CAMR Centre for Applied Microbiology & Research (ECACC, Salisbury, Wiltshire)Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000
In-Vitro Adipocytes Differentiation
expression of adipocyte-specific genes, such as fatty acid synthetase, fatty acid binding protein, leptin and adiponectin. Reagents and requirements Material and sources: DMEM (GibcoBRL-Cat# 11965-084) Calf Serum (GibcoBRL-Cat
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