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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Phosphodiesterase 5A cGMP Specific,PDE5A
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
cGMP-specific 3',5'-cyclic phosphodiesterase,PDE5A,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE, PDE5A,CGB-PDE; CN5A; PDE5; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; phosphodiesterase isozyme 5; phosphodiesterase 5A cGMP-specific
Search name
Bovine PDE5A ELISA KIT ,Bovine cGMP-specific 3' ELISA KIT ,Bovine 5'-cyclic phosphodiesterase ELISA KIT , Bovine cGMP-binding c ELISA KIT , Bovine GMP-specific phosphodiesterase ELISA KIT ,Bovine CGB-PDE ELISA KIT ,Bovine CGB-PDE ELISA KIT ,Bovine CN5A ELISA KIT ,Bovine PDE5 ELISA KIT ,Bovine cGMP-binding cGMP-specific 3' ELISA KIT ,Bovine 5'-cyclic nucleotide phosphodiesterase ELISA KIT ,Bovine cGMP-specific 3' ELISA KIT ,Bovine 5'-cyclic phosphodiesterase ELISA KIT ,Bovine cGMP-specific phosphodiesterase PDE5A2 ELISA KIT ,Bovine cGMP-specific phosphodiesterase type 5A ELISA KIT ,Bovine phosphodiesterase isozyme 5 ELISA KIT ,Bovine phosphodiesterase 5A cGMP-specific ELISA KIT
Intended use
Bovine PDE5A ELISA KIT allows for the in vitro quantitative determination of Bovine cgmp-specific 3',5'-cyclic phosphodiesterase,PDE5A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine PDE5A ELISA KIT has been pre-coated with an antibody specific to PDE5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDE5A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDE5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDE5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDE5A Related Products
> PDE5A ELISA KITS
Bovine Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
Canine Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
Human Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
Mouse Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
Rat Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
> PDE5A Antidodies
Anti PDE5A Antidody
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