- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-52494b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Neuronal-specific septin-3,SEPT3
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Neuronal-specific septin-3, SEPT3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Neuronal-specific septin-3,SEPT3,septin 3,SEP3; bK250D10.3; neuronal-specific septin 3; neuronal-specific septin-3
Search name
Bovine Neuronal-specific septin-3 ELISA KIT ,Bovine SEPT3 ELISA KIT ,Bovine septin 3 ELISA KIT ,Bovine SEP3 ELISA KIT ,Bovine bK250D10.3 ELISA KIT ,Bovine neuronal-specific septin 3 ELISA KIT ,Bovine neuronal-specific septin-3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine neuronal-specific septin-3,Neuronal-specific septin-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal-specific septin-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuronal-specific septin-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuronal-specific septin-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuronal-specific septin-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
TUNEL PROCEDURE IN BOVINE EMBRYOS
.#1901, concentration 100mg/ml as a stock. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
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