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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine DNA repair protein RAD51 homolog 1,RAD51
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT
Product Name:Bovine DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT
Packing:96T
Catalog No.:ELI-52489b
Gene Name:Bovine DNA repair protein RAD51 homolog 1, RAD51
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine DNA repair protein RAD51 homolog 1, RAD51
Alternative Name:RAD51,Bovine DNA repair protein RAD51 homolog 1, RAD51
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT allows for the in vitro quantitative determination of Bovine DNA repair protein RAD51 homolog 1, RAD51 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine DNA repair protein RAD51 homolog 1, RAD51 ELISA KIT has been pre-coated with an Bovine DNA repair protein RAD51 homolog 1, RAD51 antibody specific to Bovine DNA repair protein RAD51 homolog 1, RAD51 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine DNA repair protein RAD51 homolog 1, RAD51 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine DNA repair protein RAD51 homolog 1, RAD51, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine DNA repair protein RAD51 homolog 1, RAD51 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
同源重组(Homologus Recombination) 是指发生在姐妹染色单体(sister chromatin) 之间或同一染色体上含有同源序列的DNA分子之间或分子之内的重新组合。同源重组需要一系列的蛋白质催化,如原核生物细胞内的RecA、RecBCD、RecF、RecO、RecR等;以及真核生物细胞内的Rad51、Mre11-Rad50等等。 在人类细胞中,同源重组由Rad51介导,在有ATP的条件下,Rad51聚合在单链DNA上,形成一个核蛋白
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
/Rad51 family of proteins are the key enzymes in this homology-directed repair pathway, as they perform DNA strand invasion and exchange, in concert with a host of ancillary factors. In vitro, the RecA/Rad51 family of proteins share similar enzymatic
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