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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Proto-oncogene vav,VAV1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Proto- oncogene vav, VAV1 ELISA KIT
Product Name:Bovine Proto- oncogene vav, VAV1 ELISA KIT
Packing:96T
Catalog No.:ELI-51386b
Gene Name:Bovine Proto-oncogene vav, VAV1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Proto-oncogene vav, VAV1
Alternative Name:VAV1,Bovine Proto-oncogene vav, VAV1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Proto- oncogene vav, VAV1 ELISA KIT allows for the in vitro quantitative determination of Bovine Proto-oncogene vav, VAV1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Proto- oncogene vav, VAV1 ELISA KIT has been pre-coated with an Bovine Proto-oncogene vav, VAV1 antibody specific to Bovine Proto-oncogene vav, VAV1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Proto-oncogene vav, VAV1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Proto-oncogene vav, VAV1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Proto-oncogene vav, VAV1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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酶的活性。Intergen公司的TRAP-eze Telemerase Detection Kit在1996年率先推出。它提供特定的寡核苷酸底物,分别与底物及端粒重复序列配对的引物。如果待测样本中含有端粒酶活性,就能在底物上接上不同个数的6碱基(GGTTAG)端粒重复序列,通过PCR反应,产物电泳检测就可观察到相差六个碱基的DNA Ladder现象(参见图4)。此外,Intergen公司还提供用酶联免疫法(ELISA)检测的试剂盒. 同样,这种检测方法也不专对细胞凋亡,检测结果也不纯反应细胞凋亡的发生
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