- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-51378b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Urea transporter 1,SLC14A1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Urea transporter 1, SLC14A1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SLC14A1,SLC14A1 JK; HUT11; HsT1341; RACH1; RACH2; UT-B1; UT1; UTE; UT-B,UT11,UT3,urea transporter member 1; urea transporter JK glycoprotein; urea transporter erythrocyte; urea transporter-B1; solute carrier family 14 member 1, Urea transporter 1
Search name
Bovine SLC14A1 ELISA KIT ,Bovine SLC14A1 JK ELISA KIT ,Bovine HUT11 ELISA KIT ,Bovine HsT1341 ELISA KIT ,Bovine RACH1 ELISA KIT ,Bovine RACH2 ELISA KIT ,Bovine UT-B1 ELISA KIT ,Bovine UT1 ELISA KIT ,Bovine UTE ELISA KIT ,Bovine UT-B ELISA KIT ,Bovine UT11 ELISA KIT ,Bovine UT3 ELISA KIT ,Bovine urea transporter member 1 ELISA KIT ,Bovine urea transporter JK glycoprotein ELISA KIT ,Bovine urea transporter erythrocyte ELISA KIT ,Bovine urea transporter-B1 ELISA KIT ,Bovine solute carrier family 14 member 1 ELISA KIT ,Bovine Urea transporter 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Urea transporter 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urea transporter 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Urea transporter 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Urea transporter 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Urea transporter 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验DETECTION OF RECOMBINANT PROTEINS BY ELISA
0.5 g BSA QS to 500 ml with H 2O. ELISA Conjugate Diluent PBS, 0.05% Tween-20, 0.1% BSA, 2 µg/ml IgG) Make above ELISA diluent and add 1 mg/ml Bovine Cohn Fraction II IgG
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
Analysis of Proteins using Small Format 2D Gel Electrophoresis
-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck Ltd.)) for 5 minutes on ice
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