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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine CDC42 small effector protein 2,CDC42SE2
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine CDC42 small effector protein 2, CDC42SE2 ELISA KIT
Product Name:Bovine CDC42 small effector protein 2, CDC42SE2 ELISA KIT
Packing:96T
Catalog No.:ELI-50443b
Gene Name:Bovine CDC42 small effector protein 2, CDC42SE2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine CDC42 small effector protein 2, CDC42SE2
Alternative Name:CDC42SE2,Bovine CDC42 small effector protein 2, CDC42SE2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine CDC42 small effector protein 2, CDC42SE2 ELISA KIT allows for the in vitro quantitative determination of Bovine CDC42 small effector protein 2, CDC42SE2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine CDC42 small effector protein 2, CDC42SE2 ELISA KIT has been pre-coated with an Bovine CDC42 small effector protein 2, CDC42SE2 antibody specific to Bovine CDC42 small effector protein 2, CDC42SE2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine CDC42 small effector protein 2, CDC42SE2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine CDC42 small effector protein 2, CDC42SE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine CDC42 small effector protein 2, CDC42SE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
solution (a small spoonful of nonfat dry milk in 40 mL PBS) Bovine serum albumin (BSA) 3,3''-diaminobenzidine tetrahydrochloride (DAB; Sigma D5637) Detergent (optional, see Step 2) Drosophila (see Steps 7-9) Entellan (EMD
Analysis of Proteins using Small Format 2D Gel Electrophoresis
of the sample can be determined using a standard protein assay kit. Depending on the assay system, the lysis buffer used to prepare the bacterial proteins may give a background equivalent to approximately 5mg/ml of protein as determined from a standard curve
; TALENs; engineered TAL effector nucleases; engineered TALE nucleases; REAL; REAL?Fast; protein engineering; DNA?binding domains; FLASH GO TO THE FULL PROTOCOL: PDF
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