- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-11102b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine CDC42 small effector protein 1,CDC42SE1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine CDC42 small effector protein 1, CDC42SE1 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC42SE1,SCIP1; SPEC1; 1300002M12Rik; CDC42 small effector protein 1; CDC42-binding protein SCIP1; signaling molecule SPEC1 beta; small effector of CDC42 protein 1; small protein effector 1 of Cdc42; CDC42 small effector 1
Search name
Bovine CDC42SE1 ELISA KIT ,Bovine SCIP1 ELISA KIT ,Bovine SPEC1 ELISA KIT ,Bovine 1300002M12Rik ELISA KIT ,Bovine CDC42 small effector protein 1 ELISA KIT ,Bovine CDC42-binding protein SCIP1 ELISA KIT ,Bovine signaling molecule SPEC1 beta ELISA KIT ,Bovine small effector of CDC42 protein 1 ELISA KIT ,Bovine small protein effector 1 of Cdc42 ELISA KIT ,Bovine CDC42 small effector 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine CDC42 small effector protein 1, CDC42SE1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bovine CDC42 small effector protein 1, CDC42SE1, During the reaction, Bovine CDC42 small effector protein 1, CDC42SE1 in the sample or standard competes with a fixed amount of biotin-labeled Bovine CDC42 small effector protein 1, CDC42SE1 for sites on a pre-coated Monoclonal antibody specific to Bovine CDC42 small effector protein 1, CDC42SE1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine CDC42 small effector protein 1, CDC42SE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验two to three colonies from each agar plate and set upa small volume (typically 5 ml) LB culture for miniprep. Incubate and shake under 37 °C overnight, prepare plasmidDNA using a spin miniprep kit according to manufacturer’sprotocol. 6. (Optional) Digest
and MS/MS to identify proteins (95) or Mann and colleagues identifying proteins present after immunoprecipitation of protein complexes (124). Recently, Macara, Haystead, and coworkers used MS to identify interacting proteins with the Cdc42 effector, Borg3 (80
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
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