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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Carbonic anhydrase-related protein 11,CA11
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Carbonic anhydrase- related protein 11, CA11 ELISA KIT
Product Name:Bovine Carbonic anhydrase- related protein 11, CA11 ELISA KIT
Packing:96T
Catalog No.:ELI-50236b
Gene Name:Bovine Carbonic anhydrase-related protein 11, CA11
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Carbonic anhydrase-related protein 11, CA11
Alternative Name:CA11,Bovine Carbonic anhydrase-related protein 11, CA11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Carbonic anhydrase- related protein 11, CA11 ELISA KIT allows for the in vitro quantitative determination of Bovine Carbonic anhydrase-related protein 11, CA11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Carbonic anhydrase- related protein 11, CA11 ELISA KIT has been pre-coated with an Bovine Carbonic anhydrase-related protein 11, CA11 antibody specific to Bovine Carbonic anhydrase-related protein 11, CA11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Carbonic anhydrase-related protein 11, CA11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Carbonic anhydrase-related protein 11, CA11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Carbonic anhydrase-related protein 11, CA11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Protein Carbonyl Measurement by ELISA
) (2,3). Protein-bound DNP can also be measured with increased sensitivity by enzyme-linked immunosorbent assay (ELISA) (4,5). Samples containing protein are reacted with DNP, then the protein is nonspecifically adsorbed to an ELISA plate. Unconjugated DNP
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst. RELATED INFORMATION Technical modifications incorporated into this protocol that have enabled more reproducible patterns of banding
Substrate Tablete(antibody developed in goat) St. Louis,Mo 63178, USAAffinity isolated antibody adsorbed Lot # 043H6178With bovine horse and human serum protein Cat # 0h176Lot # 055H4835Lot # A-2179Tween 80Laboratories ,Detroit,Michigan ,USA1 drop of Tween
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