- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-50002b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Ubiquitin carboxyl-terminal hydrolase BAP1,BAP1 ELISA K
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Ubiquitin carboxyl- terminal hydrolase BAP1, BAP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
BAP1,HUCEP-13; UCHL2; hucep-6; cerebral protein 6; cerebral protein-13; BRCA1 associated protein-1
Search name
Bovine BAP1 ELISA KIT ,Bovine HUCEP-13 ELISA KIT ,Bovine UCHL2 ELISA KIT ,Bovine hucep-6 ELISA KIT ,Bovine cerebral protein 6 ELISA KIT ,Bovine cerebral protein-13 ELISA KIT ,Bovine BRCA1 associated protein-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Ubiquitin carboxyl- terminal hydrolase BAP1, BAP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin carboxyl-terminal hydrolase BAP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase BAP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase BAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin carboxyl-terminal hydrolase BAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验Studies of the Ubiquitin Proteasome System
6.3 ) Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype 20× ubiquitylation buffer 2 for E3 assays (see recipe ) Mutant ubiquitins: lysine‐less (K0), K48R, K63R, K48 only, K63 only (BioMol
A Method for Assaying Deubiquitinating Enzymes
). The purified Ub-specific protease-6 (yUBP-6) in yeast and cUBP41, Ub C-terminal hydrolase-1 (cUCH-1), cUCH-6, and cUCH-8 in chick skeletal muscle were prepared as described (13 , 15 -18 ). Ub-PESTc was purified from an E. coli strain AR13 carrying pNMHUB-PESTc
（Ubiquitin C-terminal hydrolase-L1，UCH-L1）是一种主要分布于脑内，同时对人类大脑具有高度特异性的球蛋白。 癫痫大发作组脑脊液 UCH-L1 水平显著高于部分性发作组和对照组。癫痫患者的脑脊液 UCHL1 水平与癫痫发作的严重程度和癫痫发作的持续时间密切相关。癫痫发作后 6h 内检验患者血清 UCH-L1 及 S100β 水平，结果显示癫痫组 UCHL-1 及 S100β 水平显著高于精神源性非癫痫性发作组及健康人群组，精神源性非癫痫性发作组 S100β 水平显著高于健康对照
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