- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-49715b
- 2025年07月16日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine 6.8 kDa mitochondrial proteolipid,MP68
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine 6.8 kDa mitochondrial proteolipid, MP68 ELISA KIT
Product Name:Bovine 6.8 kDa mitochondrial proteolipid, MP68 ELISA KIT
Packing:96T
Catalog No.:ELI-49715b
Gene Name:Bovine 6.8 kDa mitochondrial proteolipid, MP68
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine 6.8 kDa mitochondrial proteolipid, MP68
Alternative Name:MP68,Bovine 6.8 kDa mitochondrial proteolipid, MP68
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine 6.8 kDa mitochondrial proteolipid, MP68 ELISA KIT allows for the in vitro quantitative determination of Bovine 6.8 kDa mitochondrial proteolipid, MP68 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine 6.8 kDa mitochondrial proteolipid, MP68 ELISA KIT has been pre-coated with an Bovine 6.8 kDa mitochondrial proteolipid, MP68 antibody specific to Bovine 6.8 kDa mitochondrial proteolipid, MP68 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine 6.8 kDa mitochondrial proteolipid, MP68 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine 6.8 kDa mitochondrial proteolipid, MP68, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine 6.8 kDa mitochondrial proteolipid, MP68 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用，所需浓度尿素8-10M，盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱，溶解度为70
技术资料暂无技术资料 索取技术资料
