Bovine acetylserotonin methylt

Bovine acetylserotonin methyltransferas, ASMT ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ASMT,ASMTY; HIOMT; HIOMTY; acetylserotonin N-methyltransferase; acetylserotonin methyltransferase Y chromosome; hydroxyindole O-methyltransferase; acetylserotonin O-methyltransferase

Search name

Bovine ASMT ELISA KIT ,Bovine ASMTY ELISA KIT ,Bovine HIOMT ELISA KIT ,Bovine HIOMTY ELISA KIT ,Bovine acetylserotonin N-methyltransferase ELISA KIT ,Bovine acetylserotonin methyltransferase Y chromosome ELISA KIT ,Bovine hydroxyindole O-methyltransferase ELISA KIT ,Bovine acetylserotonin O-methyltransferase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine Acetylserotonin O-methyltransferase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetylserotonin O-methyltransferase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Acetylserotonin O-methyltransferase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acetylserotonin O-methyltransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Acetylserotonin O-methyltransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.