- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-48004b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine RAS guanyl-releasing protein 4,RASGRP4
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT
Product Name:Bovine RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT
Packing:96T
Catalog No.:ELI-48004b
Gene Name:Bovine RAS guanyl-releasing protein 4, RASGRP4
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine RAS guanyl-releasing protein 4, RASGRP4
Alternative Name:RASGRP4,Bovine RAS guanyl-releasing protein 4, RASGRP4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT allows for the in vitro quantitative determination of Bovine RAS guanyl-releasing protein 4, RASGRP4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT has been pre-coated with an Bovine RAS guanyl-releasing protein 4, RASGRP4 antibody specific to Bovine RAS guanyl-releasing protein 4, RASGRP4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine RAS guanyl-releasing protein 4, RASGRP4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine RAS guanyl-releasing protein 4, RASGRP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine RAS guanyl-releasing protein 4, RASGRP4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
Xia''Xiang Gao''Zhimin Lu''et al.(2009) FAK Phosphorylation by ERK Primes Ras-Induced Tyrosine Dephosphorylation of FAK Mediated by PIN1 and PTP-PEST.Molecular Cell''35:11–25 3.J Bossuyt''K Helmstadter''X Wu''et al.(2008) u Ca
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