- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-47979b
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine UDP-N-acetylglucosamine--dolichyl-phosphate N-acety
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DPAGT1,ALG7; CDG-Ij; CDG1J; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra; dolichyl-phosphate alpha-N-acetylglucosaminyltransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Search name
Bovine DPAGT1 ELISA KIT ,Bovine ALG7 ELISA KIT ,Bovine CDG-Ij ELISA KIT ,Bovine CDG1J ELISA KIT ,Bovine CMSTA2 ELISA KIT ,Bovine D11S366 ELISA KIT ,Bovine DGPT ELISA KIT ,Bovine DPAGT ELISA KIT ,Bovine DPAGT2 ELISA KIT ,Bovine G1PT ELISA KIT ,Bovine GPT ELISA KIT ,Bovine UAGT ELISA KIT ,Bovine UGAT ELISA KIT ,Bovine GlcNAc-1-P transferase ELISA KIT ,Bovine N-acetylglucosamine-1-phosphate transferase ELISA KIT ,Bovine UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Bovine UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Bovine dolichyl-phosphate ELISA KIT ,Bovine UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra ELISA KIT ,Bovine dolichyl-phosphate alpha-N-acetylglucosaminyltransferase ELISA KIT ,Bovine dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPAGT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DPAGT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DPAGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPAGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
尿苷二磷酸-N-Z酰葡糖胺 uridine dipho-sphate N-acetylglucosamine
简称 UDP-N-乙酰葡糖胺。系核苷酸糖的一种，由 N-乙酰萄糖胺 -1-磷酸和 UTP生物合成。对于细菌细胞的肽葡聚糖（ peptidoglycan）、酯多糖、霉菌的聚乙酰氨基葡糖、动物的透明质酸、肝素、糖蛋白、糖脂的生物合成可作为 N-乙酰葡糖胺残基的供体。此外，许多细胞具有 UDP-N-乙酰葡糖胺 -4-差向异构酶（ UDP-N-acetylglucosamine-4-epim-erase， EC5． 1． 3． 7）由于此酶的作用发生
N-乙酰葡糖胺 N-acetylglucosamine D型化学系统名称为 2-乙酰胺 -2-脱氧 -D-葡萄糖。在细菌细胞壁的胞壁质、霉菌细胞壁的几丁质，动植物细胞的糖蛋白、粘多糖等细胞或组织的支持结构物质中，以多糖组成成分而广泛分布。这些多糖用强酸水解，可脱去乙酰基游离出 D-葡糖胺。生物合成途径是通过乙酰辅酶 A将葡糖胺 -6-磷酸 N-乙酰化，进而将 N-乙酰萄糖胺 -1-磷酸转变成 UDP-N-乙酰葡糖胺，这个核苷酸糖通过糖酰基转换反应而纳入多糖链。
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