- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-47975b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine N-arachidonyl glycine receptor,GPR18
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine N- arachidonyl glycine receptor, GPR18 ELISA KIT
Product Name:Bovine N- arachidonyl glycine receptor, GPR18 ELISA KIT
Packing:96T
Catalog No.:ELI-47975b
Gene Name:Bovine N-arachidonyl glycine receptor, GPR18
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine N-arachidonyl glycine receptor, GPR18
Alternative Name:GPR18,Bovine N-arachidonyl glycine receptor, GPR18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine N- arachidonyl glycine receptor, GPR18 ELISA KIT allows for the in vitro quantitative determination of Bovine N-arachidonyl glycine receptor, GPR18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine N- arachidonyl glycine receptor, GPR18 ELISA KIT has been pre-coated with an Bovine N-arachidonyl glycine receptor, GPR18 antibody specific to Bovine N-arachidonyl glycine receptor, GPR18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine N-arachidonyl glycine receptor, GPR18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine N-arachidonyl glycine receptor, GPR18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine N-arachidonyl glycine receptor, GPR18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells
NMDA receptor is a ligand-gated ion channel receptor composed of multiple subunits encoded by at least five genes and their splice variants (1 ). Functional channels can be produced with only the NR1 subunit. These single-subunit channels
[3H](+)MK801 Radioligand Binding Assay at the N‐Methyl‐D‐Aspartate Receptor
., Siegel, B.W., Harrison, B.L., Gross, R.S., Hawes, C., and Towers, P. 1996. [3H]MDL 105,519, a high‐affinity radioligand for the N‐methyl‐D‐aspartate receptor‐associated glycine recognition site J. Pharmacol. Exp. Ther. 279:62‐68
Radioligand Binding Assays for the Glycine Site on N‐Methyl‐D‐Aspartate Receptors
. Ivanovic, A., Reilander, H., Laube, B. and Kuhse, J. 1998. Expression and initial characterization of a soluble glycine binding domain of the N‐methyl‐D‐aspartate receptor NR1 subunit. J. Biol. Chem. 273:19933
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