Bovine Myeloid- associated dif

Bovine Myeloid- associated differentiation marker- like protein 2, Myadml2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MYADML2,myeloid-associated differentiation marker-like protein 2; myeloid-associated differentiation marker-like 2

Search name

Bovine MYADML2 ELISA KIT, Bovine myeloid-associated differentiation marker-like protein 2 ELISA KIT, Bovine myeloid-associated differentiation marker-like 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Myeloid- associated differentiation marker- like protein 2, Myadml2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myadml2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myadml2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myadml2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Myadml2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.