- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-47806b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine DNA polymerase epsilon subunit 4,POLE4
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine DNA polymerase epsilon subunit 4, POLE4 ELISA KIT
Product Name:Bovine DNA polymerase epsilon subunit 4, POLE4 ELISA KIT
Packing:96T
Catalog No.:ELI-47806b
Gene Name:Bovine DNA polymerase epsilon subunit 4, POLE4
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine DNA polymerase epsilon subunit 4, POLE4
Alternative Name:POLE4,Bovine DNA polymerase epsilon subunit 4, POLE4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine DNA polymerase epsilon subunit 4, POLE4 ELISA KIT allows for the in vitro quantitative determination of Bovine DNA polymerase epsilon subunit 4, POLE4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine DNA polymerase epsilon subunit 4, POLE4 ELISA KIT has been pre-coated with an Bovine DNA polymerase epsilon subunit 4, POLE4 antibody specific to Bovine DNA polymerase epsilon subunit 4, POLE4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine DNA polymerase epsilon subunit 4, POLE4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine DNA polymerase epsilon subunit 4, POLE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine DNA polymerase epsilon subunit 4, POLE4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Rapid Separation of Protein‐Bound DNA from Free DNA Using Nitrocellulose Filters
Hinkle, D.C. and Chamberlin, M.J. 1972a. Studies of the binding of Escherichia coli RNA polymerase to DNA I. The role of sigma subunit in site selection. J. Mol. Biol. 70:157‐185
Methylated CpG island Amplification Protocol written by Minoru Toyota 2. Materials 2.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH
Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
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