- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-45681b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Ribose-phosphate pyrophosphokinase 1,PRPS1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Ribose- phosphate pyrophosphokinase 1, PRPS1 ELISA KIT
Product Name:Bovine Ribose- phosphate pyrophosphokinase 1, PRPS1 ELISA KIT
Packing:96T
Catalog No.:ELI-45681b
Gene Name:Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1
Alternative Name:PRPS1,Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Ribose- phosphate pyrophosphokinase 1, PRPS1 ELISA KIT allows for the in vitro quantitative determination of Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Ribose- phosphate pyrophosphokinase 1, PRPS1 ELISA KIT has been pre-coated with an Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1 antibody specific to Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Ribose-phosphate pyrophosphokinase 1, PRPS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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, or 1 hour at 37°C. Rinse plates 5 times with PBS-Tween. Add 50 µl per well of 1 mg/ml p-nitrophenyl phosphate substrate prepared in substrate buffer. Incubate about 1 hour to develop - WATCH CLOSELY Calibrate the ELISA plate
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